BCR ABL Detection Kits

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The BCR-ABL1 Detection Kit is intended for the qualitative detection of BCR-ABL1 fusion gene (Major-BCR, minor-BCR, and micro -BCR) in bone marrow or peripheral blood samples using real-time PCR. The kit is based on reverse transcription of total RNA, followed by real-time PCR amplification and detection of BCR-ABL1 (e13a2, e14a2 or e1a2 or e19a2) fusion transcripts and the ABL transcript.

TRUPCR® BCR-ABL1 detection is a Real-Time amplification test for the detection of BCR-ABL1 e13a2, e14a2, e1a2 and e19a2 fusion transcripts in bone marrow or peripheral blood samples. It has a two-step protocol in which total RNA is reverse-transcribed, and the generated cDNA is amplified by PCR using a pair of specific primers and a specific internal double-dye probe for BCR-ABL1 (Major, Minor and Micro) and ABL1.
To control sample quality, RNA extraction process and RT-PCR process, the kit uses the RNA detection of the gene for human ABL. Amplification of this control RNA is visualised in the FAM channel in a separate tube. This detection technology of the naturally occurring human RNA provides control of the whole diagnostic process, i.e. sample quality (sample RNA degradation), RNA extraction efficiency, reverse-transcription step efficiency and PCR amplification efficiency (PCR inhibition).

• Detection and differentiation of all three Major, Minor and Micro transcripts.
• All inclusive kit: The Assay includes cDNA preparation components and all the PCR components including PCR Pre-mix / mastermix for optimised results. No need to standardise your own cDNA prep which may lead to variability in the results.
• Detection up to 1 copy of BCR ABL1 transcript.

The TRUPCR® BCR-ABL QT kit is a RT-qPCR test for the quantitative detection of BCR-ABL fusion transcripts in bone marrow or peripheral blood samples. The kit provides an advantage by detecting, differentiating and quantifying all the three break point cluster regions i.e. major/P210 (M-bcr), minor/P190 (m-bcr) and micro/P230 (mu-bcr) in separate tubes, making it one of the most unique and comprehensive solutions currently available.

It is a two-step protocol in which total RNA from patient’s peripheral blood or bone marrow is isolated, the RT enzyme reverse transcribes total RNA and yields single-stranded cDNA. This is followed with real-time quantitative PCR amplification and quantification of BCR-ABL fusion transcripts and the ABL transcript. Three independent RT-qPCR reactions are performed to detect all the known fusion transcripts in separate tubes to differentiate, quantitate and report individual transcripts.

• The kit is calibrated to report on the International Scale using First WHO International Genetic Reference Panel for quantitation of BCR-ABL1 translocation by RQ-PCR (NIBSC code: 09/138) for harmonisation of results among laboratories.
• The measuring standards of the kit are calibrated to European reference material ERM-AD623a-f, produced and certified under the responsibility of the Institute for Reference Materials and measurements of the European Commission's Joint Research Centre.
• The kit allows highly sensitive deep molecular response reporting based on European Treatment and Outcome Study (EUTOS) guidelines.
• Detection, differentiation and quantification of all three Major, Minor and Micro transcripts.
• All inclusive kit: The Assay includes cDNA preparation components and all the PCR components including PCR Pre-mix / mastermix for optimised results. No need to standardise your own cDNA prep which may lead to variability in the results.