The TRUPCR® AMR Panel Kit is a Real-Time PCR–based nucleic acid amplification assay for the qualitative detection and differentiation of antimicrobial resistance (AMR) genes encoding resistance to Trimethoprim/Sulfamethoxazole (SXT), Extended-Spectrum Beta-Lactams (ESBLs), Carbapenems, Colistin, Vancomycin, and Methicillin from clinical samples of human origin or cultured isolates.
The assay uses fluorescent dye–labeled probes in a TaqMan® chemistry system. Each reaction includes an endogenous internal control (IC) to check for sample adequacy and possible PCR inhibition, minimizing false negatives. The kit also employs a hot-start enzyme to reduce non-specific amplification and incorporates Uracil-N-glycosylase (UNG) in the master mix to prevent carry-over contamination.
A negative result with the TRUPCR® AMR Panel Kit does not exclude the presence of other resistance mechanisms. Results must always be interpreted in conjunction with clinical and laboratory findings.
Key Features
- CE-IVD
- Detects a wide spectrum of clinically relevant AMR genes, including:
- Carbapenemases: blaKPC, blaNDM, blaVIM, blaOXA-48-like, blaIMP
- ESBLs (Extended Spectrum β-lactamases): blaCTX-M groups, blaTEM, blaSHV
- Vancomycin: vanA,vanB
- Methicillin: mecA
- Multiplex Real-time PCR with TaqMan® chemistry for high specificity and sensitivity
- Includes all reagents and positive/negative controls for the assay
- Compatible with multiple Real-Time PCR instruments:
- Applied Biosystems™ 7500 / 7500 Fast Dx
- Applied Biosystems™ QuantStudio® 3 & 5
- Rotor-Gene Q
- Bio-Rad CFX96
- Easy-to-use, reliable, and cost-effective workflow
