UPCR® MTB DNA Extraction Kit is intended for the extraction and purification of DNA from M.tuberculosis spp. in human samples (sputum, BAL, PUS, tissues and other body fluids). TRUPCR® MTB DNA Extraction Kit is based on the advanced silica binding technology. The DNA extraction procedure includes 4 steps:
Heat Lysis – Lysis of the bacterial cell wall at high temperature and release of DNA.
Binding – Adsorption of the DNA on to the membrane.
Washing – Removal of residual contaminants.
Elution – Elution of pure genomic DNA.
Key features
- CE-IVD
- Based on Silica column technology, reduces the hands on procedure time.
- TRUPCR® MTB DNA Extraction kit is validated on all clinical specimens including sputum, PUS, BAL, tissue, CSF and other body fluids.
- Phenol chloroform free method.
- High binding capacity of the column provides high yield of purified DNA.
Specification
- Technology: Silica Column
- Sample Type: Sputum, Pus, BAL, Tissue, Urine, Body fluids and tissue
- Application: PCR and Real Time PCR
- Elution Volume: 40-50 µl
- Compatible with TRUPCR® MTBC Onestep Nested kit and TRUPCR® Rifampicin Resistance MTB Detection
- Kit Processing: Manual (Centrifugation)
