Prothrombin 20210 Mutation, also called Factor II Mutation is a genetic condition that causes an increase in the likelihood of your blood forming dangerous blood clots. All individuals make the prothrombin protein that helps blood clot. However, there are certain individuals who have a DNA mutation in the gene used to make prothrombin (also called prothrombin G20210A or the factor II mutation). They are said to have an inherited thrombophilia (clotting disorder) called prothrombin G20210A. When this occurs, they make too much of the prothrombin protein.
The TRUPCR® Kit method amplifies and detects the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/adenine polymorphism in site 20210 (G20210A). Presence of the wild-type allele (G20210G) is detected in the FAM fluorescent channel and mutant allele (A20210A) in the HEX fluorescent channel. In case of heterozygous genotype (G20210A) a signal is detected in both channels. The detection kit contains ready to use master mix and takes advantage of the”hot start” technology, minimising non-specific reactions and assuring maximum sensitivity.
Key Features
- Rapid and extremely accurate test.
- Based on allele discrimination its More reliable and highly reproducible assay.
- Easy work flow & compatible with Applied Biosystems 7300 / 7500 Real-Time PCR System, AriaMx Real-Time PCR System, CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System, QuantStudio™ 3 and 5 Real-Time PCR System, Rotor-Gene Q, StepOne™ / StepOnePlus™ Real-Time PCR System.