TRUPCR® HLA-B27 PCR Kit is a Real-time PCR-based assay for the qualitative detection of HLA-B27 allele in human serum or plasma. It is based on amplification of the allelic gene region by primer and probes specific for HLA-B27. In this kit, there are two independent reactions running in parallel in each tube: the first detects HLA-B27 (FAM channel); the second detects internal control (HEX channel) which allows exclusion of unreliable results. It covers one of the highest numbers of the known allelic subtypes in comparison with other available kits in the market.
Three of the most common methods for determination of HLA antigens in clinical and research laboratories are microlymphocytotoxicity (MLCT), flowcytometry and polymerase chain reaction (PCR) but DNA testing by PCR for detection of HLA B27 is extremely accurate and considered the gold standard.
TRUPCR® HLA B27 PCR Testing Kit’s advantages:
- HLA B27 PCR tests are considered to be a gold standard.
- It is a rapid, more reliable and extremely accurate test.
- Compared to other technologies like flow cytometry, HLA B27 PCR testing does not necessarily require viable lymphoid.
- Since PCR directly detects the allele, incomplete typing is greatly reduced.
- The test is widely validated over clinical samples across national institutes, NABL and CAP-accredited labs.
About HLA B27
HLA-B27 is found in about 90% of patients with Ankylosing Spondylitis (AS). Ankylosing Spondylitis is a type of arthritis that affects the spine. PCR-based detection of HLA-B27 is extremely accurate and considered the “gold standard”.
Ankylosing Spondylitis affects about 0.1% to 0.5% of the adult population. Although it can occur at any age, spondylitis most often strikes men in their teens and 20s. Most, but not all, people with spondylitis carry a gene called HLA-B27. Although people carrying this gene are more likely to develop spondylitis, it is also found in up to 10% of people who have no signs of the condition.