Call us: +44 (0) 161 943 4731
info@trupcr.com
TRUPCR EuropeTRUPCR EuropeTRUPCR EuropeTRUPCR Europe
  • Home
  • Products
      • Oncology & Haematology
        • Individual Markers
          • C-KIT Mutation Detection Kit
          • CALR Kit
          • FLT3 Mutation Detection Kit
          • NPM1 Qualitative KIT
          • NPM1 Mutation Quantitative Kit
          • JAK2 KIT
          • JAK-2 Allele Burden Kit
          • PML-RARA Quantitative
          • PML-RARA Qualitative (BCR1, BCR2, BCR3)
        • Individual Markers BCR-ABL1
          • BCR-ABL1 b2a2/b3a2 Differentiation Kit
          • BCR-ABL Quantitative Kit
          • BCR ABL Qualitative Kit
          • BCR-ABL1 Transcript Screening Kit
        • Solid Tumours Individual Markers
          • EGFR Real-time PCR Kit
          • KRAS Real-time PCR Kit
          • MGMT Methylation Detection Kit
          • BRAF Mutation Kit
          • NRAS Mutation Kit
          • ROS1 Fusions Detection Kit
        • Panel Kits
          • ALL PCR Panel Kit
          • AML Panel Kit
          • MPN Panel Kit
          • Paediatric Leukemia Panel Kit
      • Extraction
        • Silica extraction
          • Blood DNA Extraction Kit
          • Blood RNA Extraction Kit
          • Total Viral Nucleic Acid Extraction Kit
          • Viral RNA Extraction Kit
          • MTB DNA Extraction Kit
          • Total Viral Nucleic Acid Extraction Kit
          • Tissue DNA Extraction Kit
      • Infectious Disease
        • Panel Kits
          • Influenza Panel Kit
          • Flu Panel with RSV detection Kit
          • NAAT Kit
          • Neuro Panel Kit
          • STD Panel Kit
          • Respiratory Pathogen Panel Kit
          • TORCH Panel Kit
          • Tropical Fever Panel Kit
          • UTI ID Panel Kit
        • Individual Markers: STI
          • CMV QT Kit
          • CT/NG Real-Time PCR Detection Kit
          • EBV Virus Detection Kit
          • HBV Viral Load PCR Kit
          • HCV Viral Load Kit
          • HIV Viral Load Kit
          • HCV Genotyping Kit
          • HIV 1/2 Detection Kit
          • HSV Real-time PCR Detection Kit
          • HPV High Risk (E6/E7) Kit
          • HPV 16 & 18 Kit
        • Bacterial Diseases
          • MTB Kit (Nested single step real-time PCR)
          • One Step nested Kit (MTBC and NTM)
          • Leptospira Detection Kit
        • Parasitic Diseases
          • Toxoplasma Detection Kit
        • Viral Diseases
          • BKV Viral Load Kit
          • SARS-CoV-2 RT-qPCR Kit
          • H1N1 (Swine Flu) Detection PCR Kit
        • Vector Borne Diseases
          • Zika Virus Detection Kit
          • Chikungunya Detection Kit
          • Dengue Detection kit
          • Dengue / Chikungunya PCR Kit
          • Malaria Detection Kit
      • Human Genetics
        • Human Genetics
          • Factor II Prothrombin Mutation (G20210A) Detection Kit
          • Factor V Leiden Mutation (G1691A) Detection Kit
          • MTHFR Mutation Kit
          • Coagulation/ Thrombophilia Panel qPCR Kit
          • HLA B27 PCR Kit
      • Antimicrobial Resistance
        • Panel Kits
          • UTI AST Panel Kit
        • Individual Markers
          • MRSA Detection Kit
          • Rif/INH MTB Drug Resistant Detection Kit
          • Rifampicin Resistant MTB Kit
  • Careers
  • Events
  • News
  • About us
  • Contact

TRUPCR® MTHFR Mutation Kit

The N5, N10-methylenetetrahydrofolate reductase (MTHFR) is an enzyme that plays an important role in the metabolisation of the amino acid methionine. Genetic polymorphism commonly associated with severe MTHFR deficiency is defined by a C to T substitution at position 677 (C677T) and A1298C defined by a A to C substitution at position 1298 of the MTHFR gene, which leads to the incorporation of amino acid alanine (A) instead of valine (V) at position 222 and glutamate to alanine substitution at codon 429 respectively of the MTHFR protein. The altered MTHFR is known as “Thermolabile MTHFR”. Homozygous and heterozygous carriers of these mutations both show reduced MTHFR activity. In particular, homozygous carriers suffer from significantly increased blood levels of homocysteine. In general, increased homocysteine levels are considered a risk factor of vascular diseases (e.g. arterial thrombosis). The C677T mutation in its homozygous form alone or as a compound heterozygote, which involves both C677T and an A1298C condition (where an Adenine (A) residue changes to a Cytosine (C) residue at the 1298th position) lead to the disruption of the MTHFR gene and causes a drastic reduction of the MTHFR enzyme. This in turn, leads to an elevation of Homocysteine in the blood. The TRUPCR® MTHFR Kit is an allelic discrimination real-time PCR assay for qualitative detection of the mutation. In an allelic discrimination, two different probes specific for each allele are included in the PCR assay. Each probe is labelled with a different fluorescent dye (such as FAM or HEX/VIC) at its 5’ end and contains a non fluorescent quencher at the 3’ end. During qPCR amplification of the target DNA the probes will compete for binding across the variant region.

Key Features

  • Detects Human MTHFR gene SNPs C677T and A1298C.
  • Rapid and extremely accurate test.
  • Easy work flow & compatible with Applied Biosystems 7300 / 7500 Real-Time PCR System, AriaMx Real-Time PCR System, CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System, QuantStudio™ 3 and 5 Real-Time PCR System, Rotor-Gene Q, StepOne™ / StepOnePlus™ Real-Time PCR System.

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B1331 TRUPCR® MTHFR Mutation (C677T, A1298C ) Kit 48 Reactions
3B1332 TRUPCR® MTHFR Mutation (C677T, A1298C ) Kit 96 Reactions
Back to products list

To arrange a visit or to enquire about any of our products, please feel free to contact us and one of our representatives will be in touch within 24 hours

CONTACT US

About us

With an experienced team and innovative products, TRUPCR Europe supplies high quality qPCR molecular diagnostic products throughout Europe.

We offer technical support and training on all our products and are committed to increasing the efficiency of laboratory testing and enhancing patient care.

Terms & Conditions
Privacy Policy

Quick Links

  • Home Page
  • Latest News
  • Antimicrobial Resistance
  • Extraction
  • Human Genetics
  • Infectious Disease
  • Oncology & Haematology
  • Contact us

Our Location

Unit 6, Williams House, Lloyd Street
North, Manchester Science Park,
Manchester M15 6SE

Contact Us

General enquires: info@trupcr.com
Technical support: techsupport@trupcr.com
Sales: sales@trupcr.com
Orders: orders@trupcr.com

Tel: +44 (0) 161 943 4731

 

Copyright © 2023 TRUPCR Europe Ltd - All Rights Reserved. Registered in England & Wales no. 12060630
  • Home
  • Products
    • Oncology & Haematology
      • Individual Markers
        • C-KIT Mutation Detection Kit
        • CALR Kit
        • FLT3 Mutation Detection Kit
        • NPM1 Qualitative KIT
        • NPM1 Mutation Quantitative Kit
        • JAK2 KIT
        • JAK-2 Allele Burden Kit
        • PML-RARA Quantitative
        • PML-RARA Qualitative (BCR1, BCR2, BCR3)
      • Individual Markers BCR-ABL1
        • BCR-ABL1 b2a2/b3a2 Differentiation Kit
        • BCR-ABL Quantitative Kit
        • BCR ABL Qualitative Kit
        • BCR-ABL1 Transcript Screening Kit
      • Solid Tumours Individual Markers
        • EGFR Real-time PCR Kit
        • KRAS Real-time PCR Kit
        • MGMT Methylation Detection Kit
        • BRAF Mutation Kit
        • NRAS Mutation Kit
        • ROS1 Fusions Detection Kit
      • Panel Kits
        • ALL PCR Panel Kit
        • AML Panel Kit
        • MPN Panel Kit
        • Paediatric Leukemia Panel Kit
    • Extraction
      • Silica extraction
        • Blood DNA Extraction Kit
        • Blood RNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Viral RNA Extraction Kit
        • MTB DNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Tissue DNA Extraction Kit
    • Infectious Disease
      • Panel Kits
        • Influenza Panel Kit
        • Flu Panel with RSV detection Kit
        • NAAT Kit
        • Neuro Panel Kit
        • STD Panel Kit
        • Respiratory Pathogen Panel Kit
        • TORCH Panel Kit
        • Tropical Fever Panel Kit
        • UTI ID Panel Kit
      • Individual Markers: STI
        • CMV QT Kit
        • CT/NG Real-Time PCR Detection Kit
        • EBV Virus Detection Kit
        • HBV Viral Load PCR Kit
        • HCV Viral Load Kit
        • HIV Viral Load Kit
        • HCV Genotyping Kit
        • HIV 1/2 Detection Kit
        • HSV Real-time PCR Detection Kit
        • HPV High Risk (E6/E7) Kit
        • HPV 16 & 18 Kit
      • Bacterial Diseases
        • MTB Kit (Nested single step real-time PCR)
        • One Step nested Kit (MTBC and NTM)
        • Leptospira Detection Kit
      • Parasitic Diseases
        • Toxoplasma Detection Kit
      • Viral Diseases
        • BKV Viral Load Kit
        • SARS-CoV-2 RT-qPCR Kit
        • H1N1 (Swine Flu) Detection PCR Kit
      • Vector Borne Diseases
        • Zika Virus Detection Kit
        • Chikungunya Detection Kit
        • Dengue Detection kit
        • Dengue / Chikungunya PCR Kit
        • Malaria Detection Kit
    • Human Genetics
      • Human Genetics
        • Factor II Prothrombin Mutation (G20210A) Detection Kit
        • Factor V Leiden Mutation (G1691A) Detection Kit
        • MTHFR Mutation Kit
        • Coagulation/ Thrombophilia Panel qPCR Kit
        • HLA B27 PCR Kit
    • Antimicrobial Resistance
      • Panel Kits
        • UTI AST Panel Kit
      • Individual Markers
        • MRSA Detection Kit
        • Rif/INH MTB Drug Resistant Detection Kit
        • Rifampicin Resistant MTB Kit
  • Careers
  • Events
  • News
  • About us
  • Contact
TRUPCR Europe