Human adenoviruses are medium-sized, double-stranded DNA viruses with a non-enveloped icosahedral structure. More than 50 types are known to infect humans, most often causing respiratory tract disease ranging from mild cold-like illness to more severe presentations such as pneumonia, croup, or bronchitis. Certain types can also lead to gastroenteritis, conjunctivitis, cystitis, and, in rare cases, neurological complications. Adenoviruses are hardy in the environment, persisting on surfaces and in water sources, and while infections are often self-limiting, immunocompromised individuals are at particular risk for severe or persistent disease.
The TRUPCR® Human Adenovirus Detection Kit is a real-time PCR assay intended for the qualitative detection of Human Adenovirus DNA in nucleic acids extracted from respiratory specimens. The assay uses specific primers and a dual-labelled probe system, with an internal control included in each reaction to ensure sample quality and monitor PCR inhibition.
Key Features
- Research Use Only (RUO)
- Qualitative detection of Human Adenovirus DNA from extracted respiratory specimens such as BAL, tracheal aspirate, sputum, or nasopharyngeal aspirate in VTM.
- Multiplex design with target and internal control detected in a single reaction.
- High sensitivity with reliable detection at approximately 10³ IU/mL.
- Demonstrated clinical performance with 97.5% sensitivity and 100% specificity in comparison with a CE-IVD reference method.
- Ready-to-use reagents with simplified workflow suitable for routine laboratory use.
- Compatible with widely used real-time PCR platforms, including:
- Applied Biosystems™ 7500 / 7500 Fast / QuantStudio™ 3 & 5 / StepOne™ / StepOnePlus™
- Bio-Rad CFX96 / CFX384
- Rotor-Gene Q
- Roche LightCycler® 480 II
