TRUPCR® Clostridium difficile Detection Kit is a nucleic acid amplification assay for the qualitative detection of Clostridium difficile toxin genes; Toxin A (TcdA), Toxin B (TcdB) and binary toxin (cdtA, cdtB) from extracted total nucleic acid of clinical stool specimens or rectal swabs using Real-Time PCR. An endogenous internal control (IC) is integrated into the kit to monitor PCR inhibition and specimen adequacy.
Key Features
- Research Use Only (RUO)
- Qualitative detection of tcdA, tcdB and cdtA/cdtB (binary toxin).
- Endogenous internal control included in each reaction.
- Two-tube assay format (Tube-1: Toxin A + cdtA/cdtB + IC; Tube-2: Toxin B + IC).
- Includes master mix, primer/probe mixes, positive and negative controls.
- Compatible with common real-time PCR instruments (Applied Biosystems™ 7500 Fast / QuantStudio™3/5 , Bio-Rad CFX96, Rotor-Gene, etc.).
- Store kit at -20 °C.
