The TRUPCR® Dengue, Chikungunya & Malaria Detection Kit is a multiplex real-time PCR-based assay designed for the qualitative detection of Dengue virus (DENV) RNA, Chikungunya virus (CHIKV) RNA, and Plasmodium spp. DNA from human clinical samples such as serum, plasma, and blood.
This kit enables detection of viral and parasitic targets, ensuring rapid, specific, and reliable identification of three major tropical pathogens in a single workflow.
The assay utilises one-step real-time reverse transcription PCR technology, where RNA is first reverse-transcribed into cDNA, followed by amplification and fluorescent signal detection. The integrated endogenous internal control ensures assay reliability and detects possible PCR inhibition.
Key Features
- Research Use Only (RUO)
- High sensitivity and specificity through optimized primer and probe design.
- Internal control incorporated to monitor PCR inhibition and sample quality.
- No cross-reactivity with other pathogenic virus, bacteria or fungi.
- Compatible with major real-time PCR platforms including ABI 7500, QuantStudio® 3/5, Rotor-Gene Q, Bio-Rad CFX96, and Roche LightCycler® 480 II.
- One-step RT-PCR workflow for faster and more efficient detection.
- Easy work flow & compatible with various Real-time PCR instruments allowing higher adaptability.
