TRUPCR® MTB Kit (Nested single step Real-time PCR) is an in vitro nucleic acid amplification assay for the qualitative detection of Mycobacterium tuberculosis complex (MTBC). It is an assay that utilizes two sequential polymerase chain reactions to detect low load of Mycobacterium DNA in human clinical samples, both pulmonary and extra- pulmonary. Each test is a multiplex reaction with three independent reactions running in parallel in which two targets are for Mycobacterium tuberculosis complex (MTBC) detection in Hex/Yellow channel and one for internal control (IC) detection in Tex Red/orange channel.
Key Features
- Dual target for rapid and accurate detection of MTBC infection.
- Highly sensitive assay due to one step, single tube nested PCR format.
- Takes advantage of the “hot start” technology to minimizing non-specific reactions and assuring maximum sensitivity & specificity.
- Takes advantage of the uracil DNA glycosylase (UDG) in Master mix to eliminate possible contamination of the run with amplification products.
- Validated on pulmonary & various extra pulmonary samples.
- Internal Control included in the kit.
- CE-IVD certified kit.
- Limit of detection (LOD) is at the level of 10 CFU/ml.
- Easy work flow & compatible with various Real-time PCR instruments and also available for end-point PCR gel based detection.
- Available in different pack sizes.
Click here to access the kit insert/IFU and the Declaration of Conformity for this product.
