The TRUPCR® dPCR JAK2 V617F Kit is a highly sensitive digital PCR assay developed for the absolute quantification of the JAK2 V617F somatic mutation (c.1849G>T) located in exon 14 of the JAK2 gene. The assay is designed for use with genomic DNA extracted from bone marrow or peripheral blood samples and is optimised for the Bio-Rad Droplet Digital™ PCR System.
Key Features of the TRUdPCR JAK2 V617F Kit:
- Built on advanced droplet digital PCR (dPCR) technology, partitioning samples into thousands of micro-reactions for precise detection of mutant and wild-type JAK2 sequences.
- Provides accurate absolute quantification without requiring standard curves, delivering high sensitivity, specificity, and reproducibility.
- Uses hydrolysis probe chemistry with mutation-specific fluorescent probes to generate targeted fluorescence signals during amplification.
- Enables clear differentiation between positive and negative reactions, supporting reliable detection of low-frequency JAK2 mutations in research samples.
TRUPCR® dPCR JAK2 V617F Kit is intended strictly for Research Use Only (RUO). It is not intended for diagnostic procedures or clinical decision-making.
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