TRUPCR® BCR-ABL QT kit is a RT-qPCR test for the quantitative detection of BCR-ABL fusion transcripts in bone marrow or peripheral blood samples. The kit provides an advantage by detecting, differentiating and quantifying all the three break point cluster regions i.e. major/P210 (M-bcr), minor/P190 (m-bcr) and micro/P230 (mu-bcr) in separate tubes, making it one of the most unique and comprehensive solution currently available.
Principle and Procedure
It is a two-step protocol in which total RNA from patient’s peripheral blood or bone marrow is isolated, the RT enzyme reverse transcribes total RNA and yields single-stranded cDNA. This is followed with real-time quantitative PCR amplification and quantification of BCR-ABL fusion transcripts and the ABL transcript. Three independent RT-qPCR reactions are performed to detect all the known fusion transcripts in separate tubes to differentiate, quantitate and report individual transcripts.