DPYD Genetic Mutation Detection Kit
The CE-IVD TRUPCR® DPYD Testing Kit is an in vitro diagnostic test intended for the detection of clinically relevant single nucleotide polymorphisms (SNPs); c.1905+1G>A (rs3918290, also IVS14+1G>A, allele 2A*), c.1679T>G (rs55886062, allele 13*), c.2846A>T (rs67376798) and c.1129–5923C>G (rs75017182, HapB3) in dihydropyrimidine dehydrogenase (DPD) enzyme encoding gene DPYD from human genomic DNA, extracted from blood/bone marrow.
The DPYD Flyer can be downloaded here
Why DPYD Testing Is Important
Fluoropyrimidines such as 5-fluorouracil (5-FU), capecitabine and tegafur are commonly used drugs for treating solid tumours. These drugs are metabolized in the liver by enzyme dihydropyrimidine dehydrogenase (DPD) which inactivates more than 80% of these drugs. Certain single point mutations in the gene encoding this enzyme, DPYD can result in no/reduced activity of the enzyme leading to toxicity. Spectrum of severity of toxicity varies wherein patients with decreased DPD activity may experience diarrhoea, nausea, vomiting, may require hospitalization and dose reduction, patients with complete lack of DPD activity may experience life-threatening toxicity and need to be removed from the therapy.
A more detailed overview of DPYD testing can be found here
DPYD Testing Kit Principles and Procedure:
The TRUPCR® DPYD Testing Kit is based on allele-specific amplification and is achieved by ARMS PCR. The detection of the SNPs is achieved in multiplex reactions using fluorescent probes labelled with FAM/HEX or TEXAS RED where FAM/HEX inform about the SNP of interest, TEXAS RED acts as an internal reference. The internal reference is the part of the DPYD gene with no known mutation.
Key Features of the TRUPCR® DPYD Testing Kit:
- CE-IVD
- The DPYD Testing Kit allows highly sensitive and specific diagnosis of DPYD Mutations in single run.
- The kit offers sensitivity to detect as low as 10ng of DNA.
- Sample Type – Human genomic DNA, extracted from whole blood/bone marrow/FFPE tissue
- Our DPYD Testing Kit is an all-inclusive solution – The Assay includes all the PCR components including PCR Pre-mix / mastermix for optimized results.
- Positive control is included in the kit for reliable results.
- Compatible Instruments – Applied Biosystems™ 7500 series / QuantStudio® series, Bio-Rad CFX96, AriaMx Real-Time PCR, Roche LightCycler® 480-II, Linegene K Real-Time PCR
Extended Version
- Includes c.557A>G (Y186C), c.868A>G (K290E), and c.2279C>T (T760I), all associated with decreased enzyme activity.
DPYD interpretation software
The RUO TRUPCR interpretation software simplifies and accelerate results analysis:
- Experiment Overview and Issue Highlighting: The software provides a comprehensive overview of experiments in a tabular format, automatically identifying and highlighting any issues encountered during the experiment.
- Automated Report Generation: Final reports are generated for all samples in the experiment, including amplification curves for visual verification, eliminating the need for manual checking.
- Improper Amplification Detection: The software uses machine learning algorithms to flag improper amplifications, which may result from incorrect threshold settings, ensuring accurate and automated data interpretation.
