The TRUPCR® JAK-2 V617F Mutation Detection Kit is a real-time polymerase chain reaction (PCR) assay for quantification of V617F mutation in JAK-2 (Janus Kinase 2) gene against a background of wild-type genomic DNA. This mutation is a guanine to thymidine transversion in position 1849 of the JAK-2 gene, which leads to a valine to phenylalanine substitution in position 617 of the protein (V617F). The test utilizes mutation-specific primers and a JAK-2 V617F/G1849T targeted fluorescent probe to detect low-copy number (1%) JAK-2 V617F-mutant DNA in cancer tissue.
The TRUPCR JAK-2 V617F Mutation Detection Kit is a highly selective and sensitive assay for quantification of V617F mutation in the JAK-2 oncogene.