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TRUPCR® JAK-2 Allele Burden Kit

TRUPCR JAK-2 allele burden kit is a real-time polymerase chain reaction (PCR) assay for quantification V617F mutation in JAK-2 (Janus Kinase 2) gene against a background of wild-type genomic DNA. This mutation is a guanine to thymidine transversion in position 1849 of the JAK-2 gene, which leads to a valine to phenylalanine substitution in position 617 of the protein (V617F). The test utilizes mutation-specific primers and a JAK-2 V617F/G1849T targeted fluorescent probe to detect low-copy number (1%) JAK-2 V617F-mutant DNA in cancer tissue.

The TRUPCR JAK-2 V617F allele burden Kit is a highly selective and sensitive assay for quantification of V617F mutation in the JAK-2 oncogene.

Key Features

  • The test allows highly sensitive quantification up to 1% mutant sequence copies in a background of 99% wild-type DNA.
  • High specificity due to site-specific hydrolysis probes utilized in the reaction.
  • Quantitative standards are calibrated to WHO 1st International Reference Panel for Genomic JAK2 V617F mutation.
  • Easy workflow & compatible with most of the Real-time PCR equipment.
  • Available in different pack sizes.

More About JAK-2

The Janus kinase 2 gene (JAK-2) codes for a tyrosine kinase (JAK-2) that is associated with the cytoplasmic portion of a variety of transmembrane cytokine and growth factor receptors important for signal transduction in hematopoietic cells. Signalling via JAK-2 activation causes phosphorylation of downstream signal transducers and activators of transcription (STAT) proteins (eg, STAT5) ultimately leading to cell growth and differentiation.

Chronic myeloproliferative neoplasms (MPNs) are clonal hematopoietic stem cell malignancies characterized by excessive production of blood cells. BCR-ABL1-negative MPN frequently harbour an acquired single nucleotide mutation in JAK-2 characterized as c.G1849T; p. Val617Phe (V617F) and it is a gain-of-function mutation that leads to clonal proliferation. The JAK-2 V617F is present in 95% to 98% of polycythaemia vera (PV), and 50% to 60% of primary myelofibrosis (PMF) and essential thrombocythemia (ET). It has also been described infrequently in other myeloid neoplasms, including chronic myelomonocytic leukaemia and myelodysplastic syndrome. Diagnostic criteria for ET, MF, and PV adopted by the World Health Organization (WHO) include identification of a clonal marker, with a specific recommendation to test for the JAK-2 V617F mutation in exon 14.1 Detection of the JAK-2 V617F is useful to help establish the diagnosis of MPN and The JAK-2 allele burden decreases with successful therapy, disappears in some patients, and reappears during relapse.

References

  • Tefferi A, Vardiman JW. Classification and diagnosis of myeloproliferative neoplasms: the 2008 World Health Organization criteria and point-of-care diagnostic algorithms. Leukemia. 2008;22:14-22.
  • Kiladjian JJ, Cassinat B, Turlure P, et al. High molecular response rate of polycythemia vera patients treated with pegylated interferon α-2a. Blood. 2006;108:2037-2040.
  • Kröger N, Badbaran A, Holler E, et al. Monitoring of the JAK2-V617F mutation by highly sensitive quantitative real-time PCR after allogeneic stem cell transplantation in patients with myelofibrosis. Blood. 2007;109:1316-1321.
  • Jerald Z. Gong, et al. Laboratory Practice Guidelines for Detecting and Reporting JAK-2 and MPL Mutations in Myeloproliferative Neoplasms. J Mol Diagn 2013, 15: 733e744.

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B1248 TRUPCR® JAK2 allele burden kit 24 Reactions
3B1249 TRUPCR® JAK2 allele burden kit 48 Reactions
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  • Home
  • Products
    • Oncology & Haematology
      • Individual Markers
        • C-KIT Mutation Detection Kit
        • CALR Kit
        • FLT3 Mutation Detection Kit
        • NPM1 Qualitative KIT
        • NPM1 Mutation Quantitative Kit
        • JAK2 KIT
        • JAK-2 Allele Burden Kit
        • PML-RARA Quantitative
        • PML-RARA Qualitative (BCR1, BCR2, BCR3)
      • Individual Markers BCR-ABL1
        • BCR-ABL1 b2a2/b3a2 Differentiation Kit
        • BCR-ABL Quantitative Kit
        • BCR ABL Qualitative Kit
        • BCR-ABL1 Transcript Screening Kit
      • Solid Tumours Individual Markers
        • EGFR Real-time PCR Kit
        • KRAS Real-time PCR Kit
        • MGMT Methylation Detection Kit
        • BRAF Mutation Kit
        • NRAS Mutation Kit
        • ROS1 Fusions Detection Kit
      • Panel Kits
        • ALL PCR Panel Kit
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        • Paediatric Leukemia Panel Kit
    • Extraction
      • Silica extraction
        • Blood DNA Extraction Kit
        • Blood RNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Viral RNA Extraction Kit
        • MTB DNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Tissue DNA Extraction Kit
    • Infectious Disease
      • Panel Kits
        • Influenza Panel Kit
        • Flu Panel with RSV detection Kit
        • NAAT Kit
        • Neuro Panel Kit
        • STD Panel Kit
        • Respiratory Pathogen Panel Kit
        • TORCH Panel Kit
        • Tropical Fever Panel Kit
        • UTI ID Panel Kit
      • Individual Markers: STI
        • CMV QT Kit
        • CT/NG Real-Time PCR Detection Kit
        • EBV Virus Detection Kit
        • HBV Viral Load PCR Kit
        • HCV Viral Load Kit
        • HIV Viral Load Kit
        • HCV Genotyping Kit
        • HIV 1/2 Detection Kit
        • HSV Real-time PCR Detection Kit
        • HPV High Risk (E6/E7) Kit
        • HPV 16 & 18 Kit
      • Bacterial Diseases
        • MTB Kit (Nested single step real-time PCR)
        • One Step nested Kit (MTBC and NTM)
        • Leptospira Detection Kit
      • Parasitic Diseases
        • Toxoplasma Detection Kit
      • Viral Diseases
        • BKV Viral Load Kit
        • SARS-CoV-2 RT-qPCR Kit
        • H1N1 (Swine Flu) Detection PCR Kit
      • Vector Borne Diseases
        • Zika Virus Detection Kit
        • Chikungunya Detection Kit
        • Dengue Detection kit
        • Dengue / Chikungunya PCR Kit
        • Malaria Detection Kit
    • Human Genetics
      • Human Genetics
        • Factor II Prothrombin Mutation (G20210A) Detection Kit
        • Factor V Leiden Mutation (G1691A) Detection Kit
        • MTHFR Mutation Kit
        • Coagulation/ Thrombophilia Panel qPCR Kit
        • HLA B27 PCR Kit
    • Antimicrobial Resistance
      • Panel Kits
        • UTI AST Panel Kit
      • Individual Markers
        • MRSA Detection Kit
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