TRUPCR® NPM1 Mutation Quantitative Kit is an in vitro nucleic acid amplification assay for the qualitative detection and quantification of NPM1 mutant allelic burden in clinical samples. In this assay, extracted RNA is subjected to a separate Real-time reverse transcription-polymerase chain reaction (RT-PCR) procedures to detect and quantitate NPM1 mutations (mutA, mutB and mutD) in exon 12 by Real-time PCR. An additional amplification for the ABL1 gene is performed as a control for sample RNA quality and as a reference for relative quantification.
The three types of NPM1 mutation variants quantified by this kit are given below:
- NPM1 mutation A (c.860_863dupTCTG)
- NPM1 mutation B (c.863_864insCATG)
- NPM1 mutation D (c.863_864insCCTG)
Key Features:
- Accurate and specific quantification of NPM1 A, B, and D mutations
- Sensitivity down to 10 copies of mutant transcripts
- Includes all reagents required for cDNA synthesis and Real‑time PCR
- Compatible with a wide range of Real‑time PCR instruments
- Easy‑to‑use, rapid, reliable, comprehensive, and cost‑effective testing workflow
The NPM1 Quantitative interpretation software
The RUO TRUPCR interpretation software simplifies and accelerates results analysis:
- Experiment Overview and Issue Highlighting: The software provides a comprehensive overview of experiments in a tabular format, automatically identifying and highlighting any issues encountered during the experiment.
- Automated Report Generation: Final reports are generated for all samples in the experiment, including amplification curves for visual verification, eliminating the need for manual checking.
- Improper Amplification Detection: The software uses machine learning algorithms to flag improper amplifications, which may result from incorrect threshold settings, ensuring accurate and automated data interpretation.
- Initial Copy Number Validation: It checks for the correct entry of initial copy numbers, helping to reduce human errors.
