Test Overview
TRUPCR® PML-RARA BCR3 Standards Kit is designed to enable accurate calibration and quantification of the PML RARA-BCR3 fusion gene in RT-qPCR workflows. Precisely defined standards are provided for producing reliable standard curves, allowing consistent and reproducible control gene measurement.
The kit contains six plasmid-based standards with tenfold serial dilutions ranging from 10⁶ to 10¹ copies. The standards are supplied as ready-to-use aliquots and are intended for construction of a full calibration curve, supporting robust assay performance, accurate quantification, and reliable normalisation.
Principle and Application
The TRUPCR® PML RARA-BCR3 Standards Kit is used in conjunction with RT-qPCR assays to establish a standard curve for quantifying PML RARA-BCR3 fusion transcripts. The defined serial dilutions allow precise determination of target copy number in test samples.
Six standards (10⁶ to 10¹ copies per 5 µL) are used to generate a tenfold dilution series for calibration. Each dilution is recommended to be run in duplicate to ensure high-confidence results and assay reproducibility.
This kit is intended for research use only and is designed for use alongside dedicated primer and probe sets for PML RARA-BCR3 detection in RT-qPCR workflows. It supports accurate fusion transcript analysis and assay validation in molecular research applications.
