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TRUPCR® PML-RARA Quantitative

TRUPCR® PML-RARA is an in vitro nucleic acid amplification assay for the qualitative and quantitative detection of PML/RAR-alpha fusion transcripts in human clinical samples. In this multi-tube assay, extracted RNA is subjected to a separate Real-time reverse transcription-polymerase chain reaction (RT-PCR) procedures to detect and quantitate long (L or bcr1), variant (V or bcr2) and short (S or bcr3) isoforms simultaneously. An additional amplification for the ABL gene is performed as a control for sample RNA quality and as a reference for relative quantification.

Key Features:

  • First commercial assay to accurately detect, differentiate and quantify bcr1, bcr2 & bcr3 fusion transcripts.
  • Detection and differentiation of both 5’ and 3’ forms of bcr2 (variant) form.
  • Higher sensitivity and specificity with easy workflow and quick analysis.
  • All the reagents required for the test included in the kit.
  • Compatible with various real time PCR instruments.

Detection of the PML/RARA t(15;17) translocation is diagnostic for acute promyelocytic leukaemia (APL), although the diagnosis can also be based on morphology. Investigations suggest that 99% of APL patients harbour a translocation between chromosomes 15 and 17, which fuses the retinoic acid receptor alpha (RARA) gene on chromosome 17 with the PML gene on chromosome 15. Detection of the PML/RARA t(15;17) translocation is therefore used within clinical research as an identifier for APL. Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed. They represent 50-55%, 5-10% and 30-40% of the cases respectively.

The presence of this translocation is necessary for response to all-trans-retinoic acid and arsenic trioxide. Thus, the PML/RARA t(15;17) assay is useful for diagnosis and predicting treatment response. It is also helpful for monitoring therapeutic response and MRD and for detecting early relapse.

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B1259 TRUPCR® PML/RARA Quantitative Kit 24 Reactions
3B1260 TRUPCR® PML/RARA Quantitative Kit 48 Reactions
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We offer technical support and training on all our products and are committed to increasing the efficiency of laboratory testing and enhancing patient care.

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  • Home
  • Products
    • Oncology & Haematology
      • Individual Markers
        • C-KIT Mutation Detection Kit
        • CALR Kit
        • FLT3 Mutation Detection Kit
        • NPM1 Qualitative KIT
        • NPM1 Mutation Quantitative Kit
        • JAK2 KIT
        • JAK-2 Allele Burden Kit
        • PML-RARA Quantitative
        • PML-RARA Qualitative (BCR1, BCR2, BCR3)
      • Individual Markers BCR-ABL1
        • BCR-ABL1 b2a2/b3a2 Differentiation Kit
        • BCR-ABL Quantitative Kit
        • BCR ABL Qualitative Kit
        • BCR-ABL1 Transcript Screening Kit
      • Solid Tumours Individual Markers
        • EGFR Real-time PCR Kit
        • KRAS Real-time PCR Kit
        • MGMT Methylation Detection Kit
        • BRAF Mutation Kit
        • NRAS Mutation Kit
        • ROS1 Fusions Detection Kit
      • Panel Kits
        • ALL PCR Panel Kit
        • AML Panel Kit
        • MPN Panel Kit
        • Paediatric Leukemia Panel Kit
    • Extraction
      • Silica extraction
        • Blood DNA Extraction Kit
        • Blood RNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Viral RNA Extraction Kit
        • MTB DNA Extraction Kit
        • Total Viral Nucleic Acid Extraction Kit
        • Tissue DNA Extraction Kit
    • Infectious Disease
      • Panel Kits
        • Influenza Panel Kit
        • Flu Panel with RSV detection Kit
        • NAAT Kit
        • Neuro Panel Kit
        • STD Panel Kit
        • Respiratory Pathogen Panel Kit
        • TORCH Panel Kit
        • Tropical Fever Panel Kit
        • UTI ID Panel Kit
      • Individual Markers: STI
        • CMV QT Kit
        • CT/NG Real-Time PCR Detection Kit
        • EBV Virus Detection Kit
        • HBV Viral Load PCR Kit
        • HCV Viral Load Kit
        • HIV Viral Load Kit
        • HCV Genotyping Kit
        • HIV 1/2 Detection Kit
        • HSV Real-time PCR Detection Kit
        • HPV High Risk (E6/E7) Kit
        • HPV 16 & 18 Kit
      • Bacterial Diseases
        • MTB Kit (Nested single step real-time PCR)
        • One Step nested Kit (MTBC and NTM)
        • Leptospira Detection Kit
      • Parasitic Diseases
        • Toxoplasma Detection Kit
      • Viral Diseases
        • BKV Viral Load Kit
        • SARS-CoV-2 RT-qPCR Kit
        • H1N1 (Swine Flu) Detection PCR Kit
      • Vector Borne Diseases
        • Zika Virus Detection Kit
        • Chikungunya Detection Kit
        • Dengue Detection kit
        • Dengue / Chikungunya PCR Kit
        • Malaria Detection Kit
    • Human Genetics
      • Human Genetics
        • Factor II Prothrombin Mutation (G20210A) Detection Kit
        • Factor V Leiden Mutation (G1691A) Detection Kit
        • MTHFR Mutation Kit
        • Coagulation/ Thrombophilia Panel qPCR Kit
        • HLA B27 PCR Kit
    • Antimicrobial Resistance
      • Panel Kits
        • UTI AST Panel Kit
      • Individual Markers
        • MRSA Detection Kit
        • Rif/INH MTB Drug Resistant Detection Kit
        • Rifampicin Resistant MTB Kit
  • Careers
  • Events
  • News
  • About us
  • Contact
TRUPCR Europe