Test Overview
The TRUPCR® ROS1 Gene Fusions Detection Kit is intended for the qualitative detection of 14 ROS1 gene fusions in human NSCLC formalin-fixed paraffin- embedded (FFPE) tissue samples using real time PCR system. The human ABL1 gene serves as an internal control for human nucleic acid, also included in this kit.
Principle and Procedure
It has two-step protocol in which total RNA is reverse- transcribed, and the generated cDNA is amplified by PCR using a pair of specific primers and a specific internal double-dye probe of ROS1 and endogenous control gene ABL1 to monitor the quality and quantity of extracted RNA.
